Journal: Biomedicines

Article Title: EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling

doi: 10.3390/biomedicines10040794

Figure Lengend Snippet: Details on glioma samples. Indicated are details on analyzed samples including age, sex, EGFR analysis, and molecular genetic hallmarks. n.a.: not available, wt: wildtype, u unmethylated, m: methylated, d: deleted, n: not deleted.

Article Snippet: In summary, our findings demonstrate that Infinium EPIC Bead Chip analysis that is routinely applied in molecular brain tumor classification [ ] is a reliable technique for detecting EGFR amplifications compared with standard FISH analysis.

Techniques: Methylation, Biomarker Discovery, Mutagenesis

Journal: Biomedicines

Article Title: EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling

doi: 10.3390/biomedicines10040794

Figure Lengend Snippet: Detection of EGFR copy numbers in gliomas. Analysis of 34 glioma samples showed that it is possible to distinguish between EGFR non-amplification (e.g., sample T34) ( a – d ) and EGFR amplification (e.g., sample T25) ( e – h ) using FISH ( b , f ) and EPIC analysis ( c , g ). These findings also correlate with protein expression levels using IHC ( d , h ). HE: Hematoxylin–Eosin; FISH: fluorescence in situ hybridization; EPIC: Illumina Infinium EPIC Bead Chip; IHC: immunohistochemistry. Scale bars: 50 µm ( a , d , e , h ), 10 µm ( b , f ).

Article Snippet: In summary, our findings demonstrate that Infinium EPIC Bead Chip analysis that is routinely applied in molecular brain tumor classification [ ] is a reliable technique for detecting EGFR amplifications compared with standard FISH analysis.

Techniques: Amplification, Expressing, Fluorescence, In Situ Hybridization, Immunohistochemistry

Journal: Biomedicines

Article Title: EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling

doi: 10.3390/biomedicines10040794

Figure Lengend Snippet: Integrated analysis of EGFR amplification and molecular genetic hallmarks of glioma. Correlation of EGFR amplification and IDH status showed EGFR amplification in IDH wildtype gliomas using both FISH ( a ) and EPIC analysis ( b ) ( p > 0.05, Student’s t -test), with 26% of IDH wildtype gliomas being EGFR amplified ( c ) and no EGFR amplified case in IDH mutated gliomas ( d ). In the case of TERT mutation status, we found that EGFR amplification occurs in TERT mutated cases using FISH ( e ) and EPIC analysis ( f ) ( p > 0.05, Student’s t-test), with no EGFR amplified case in TERT wildtype gliomas ( g ) and 27% of EGFR amplified cases in TERT mutated gliomas ( h ). Analysis of EGFR amplification and MGMT methylation status showed no differences in EGFR amplification in MGMT methylated and unmethylated gliomas using FISH ( i ) and EPIC analysis ( j ) ( p > 0.05, Student’s t-test), 19% of MGMT methylated cases ( k ), and 22% of MGMT unmethylated cases showing EGFR amplification ( l ). In terms of LOH 1p/19q EGFR amplification was found in gliomas without LOH 1p/19q using FISH ( m ) and EPIC analysis ( n ) ( p > 0.05, Student’s t -test), with 11% of cases without LOH 1p/19q ( o ) and no case with LOH 1p/19q being EGFR amplified ( p ). wt: wildtype; m: methylated; u: unmethylated; LOH: loss of heterozygosity.

Article Snippet: In summary, our findings demonstrate that Infinium EPIC Bead Chip analysis that is routinely applied in molecular brain tumor classification [ ] is a reliable technique for detecting EGFR amplifications compared with standard FISH analysis.

Techniques: Amplification, Mutagenesis, Methylation